Protocols
From IGGP
This page provides protocols by individuals. A discussion has started to develop standardized protocols and recommendations, which are under development. Please cite the appropriate publications if using any of these protocols.
DNA extraction
Hydroponics
See Wheatley et al. 2009 Am. J. Enol. Vitic. 60:4:542-549
Metabolite extraction
Protein extraction
See Vincent et al. 2006 Electrophoresis 27:1853-1865
RNA extraction Grape tissues are difficult to extract. Many people have struggled with RNA degradation in their grape samples. The Cramer lab investigated many protocols, most of which were not suitable. The work is summarized in the following publication (Tattersall et al. 2005 Am J Enol Vitic 56:400-406). Note there is a published error in the TRIS-LiCl method. It should read 10 mM EDTA not 300 mM EDTA. See the TRIS-LiCl RNA extraction protocol below for the correct methodology.
The Cramer RNA extraction protocols are based on the publication: Tattersall et al. 2005 Am J Enol Vitic 56:400-406
- TRIS-LiCl RNA extraction (Contact Grant Cramer email for any questions)
- RNeasy RNA extraction (Contact Grant Cramer email for any questions)
- RNA extraction used by Qiu Laboratory (Contact Wenping Qiu email for any questions)
Transcript quantification
Real-Time RT-PCR
Transformation
- Step by Step Instructions for Biolistic Transformation (Contact José Vidal email for any questions)
This page is monitored by Grant Cramer
